Fluorescence: Principles and Applications to the Characterization of Macromolecular Conformations. |  |
Prof. Jean Duhamel University of Waterloo, Canada |
Course Outline:
The main appeal of fluorescence in Macromolecular Science is that it can be applied to quantitatively characterize the conformation of individual macromolecules in solution due to its extreme sensitivity with working concentrations of fluorescently labeled macromolecules in the range of 5 mg/L, 3 orders of magnitude lower than any other technique aiming to characterize the conformation of macromolecules in solution. As an introduction, the course will cover the fluorescence phenomenon followed by the description of the steady-state and time-resolved fluorometers, fluorescence resonance energy transfer (FRET), fluorescence quenching, fluorescence anisotropy, and the analysis of fluorescence decays.
- Introduction
- Instrumentation: Steady-state and time-resolved fluorometers
- Techniques
- Fluorescence resonance energy transfer
- III.B Fluorescence quenching
- III.C Fluorescence Anisotropy
- Analysis of fluorescence decays